Chloroplast Ribosome Biogenesis in Chlamydomonas
نویسندگان
چکیده
Chloroplast protein synthesis in Chlamydomonas reinhardtii is dispensable when cells are provided acetate as a carbon source. Mutants defective in synthesis, assembly, or function of chloroplast ribosomes are therefore conditionally viable. Positive selection of nonphotosynthetic cells on arsenate has been combined with a simple screening procedure to isolate mutants with a broad spectrum of defects in chloroplast protein synthesis. Eight new mutants deficient in chloroplast ribosomes have been isolated. Three of these have been characterized genetically and phenotypically, and compared with two previously described ribosome mutants, ac-20 and cr-1. A working model of ribosome assembly is proposed which suggests possible biochemical roles for these five Mendelian gene loci.
منابع مشابه
THE EFFECTS OF INHIBITORS OF RNA AND PROTEIN SYNTHESIS ON THE RECOVERY OF CHLOROPLAST RIBOSOMES, MEMBRANE ORGANIZATION, AND PHOTOSYNTHETIC ELECTRON TRANSPORT IN THE ac-20 STRAIN OF CHLAMYDOMONAS REINHARDI
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عنوان ژورنال:
- The Journal of Cell Biology
دوره 63 شماره
صفحات -
تاریخ انتشار 1974